High Performance Liquid Chromatography (HPLC) for Pharmaceutical Analysis 2010
Dr. Shulamit Levin
Medtechnica
Email at Medtechnica
Private Email
Homepage:  http://www.forumsci.co.il/HPLC

Day 1: Discovery & Research

1-1-Modes of Chromatography: Expansion

Reversed Phase

Ion-Exchange (biomolecules)

Size Exclusion (polymers)

-2-High Throughput Chromatography:

U-HPLC

1-3-Biopharmaceutical Chromatography

Detectors for Drug Discovery (PDA, MS, FLR)

Sample preparation by SPE

LC-MS/MS overview

Day 2: Development: Chemical Synthesis and Formulation

2-1-Quantitative Work using HPLC-Part 1:

Calibration and quantitation

Principles of method validation

2-2-Stability Indicating Methods

Peak Purity and Spectral Matching by PDA and MS

2-3-Separating complex Mixtures

Working with Gradients

Day 3: Manufacturing: QC of API and Dosage Forms

3-1-Quantitative work by HPLC part 2:

System Suitability principles

Integration events

Extraneous peaks in the chromatogram (System Peaks)

3-2-Detection qualification:

Sensitivity, Selectivity, Dynamic range, etc

3-3-Preparative Chromatography:

Scaling up and overloading; Purification of major component; Isolation of minor components