Solid
Phase Extraction (SPE) 
Hebrew
presentation
Sample Preparation
Book
(Register
first to Waters' site here)
Presentation
- Introduction
Oasis
Applications Notebook
Sample
preparation SPE search for application
protocol
96
well solid phase extraction
Scaling
Up-Preparative Chromatography
גימלון להפרדות פרפרטיביות
(login to website first:
http://www.waters.com)
Preparative
chromatography Considerations- by
Waters
Presentation-html
Course
handouts on Preparative
Chromatography
Scale
up of Peptide Separations
Study
of Selectivity and Loadability of
Pharmaceutical Compounds on RPLC
Columns
Preparative
Loadability as a Function of pH
Preparative Chromatography at High pH
The
Role of the Column in Preparative HPLC
Practical
Aspects of Preparative HPLC in
Pharmaceutical and Development
Production
Scaling
Down מיזעור לכרומטוגרפיה מהירה או
ננו
Ultra
Performance Liquid Chromatography
(UPLC): An IntroductionUltra
Performance Liquid Chromatography -
sub 2 micron columns
Separation
sciences redifined
LITERATURE
ספרות מקצועית:
Free
pdf Chromatography books: LC and GC
ספרים אלקטרוניים בחינם
BookRags-on-line free books:
HPLC
i-Papers
by SCRIBD מאמרים וכתבים בחינם
Chromatographic
Science Series
Textbooks
Journals
Special Topics
Abstracts
Wiley's News in
Separation Sciences
Text Books
Amazon:
BOOKS ON CHROMATOGRAPHY
Chromatographic
Science Books
Analytical
Chemistry in a GMP Environment
My Favorite
Electronic Library הספריה
האלקטרונית שלי
Magazines
on-line מגזינים מקצועיים
Chemistry
Journals כתבי עת בכימיה
Wiley InterScience
http://www3.interscience.wiley.com/search/allsearch
ScienceDirect
http://www.sciencedirect.com/science/publications/journal/chemistry
Beilstein Abstracts @
ChemWeb.com
http://www.chemweb.com/
Scirus
http://www.scirus.com/
CAS - Chemical
Abstracts Service
http://www.cas.org
ACS publications
http://pubs.acs.org/journals/query/nonsubscriberSearch.jsp
ISI - Thomson
http://www.isiwebofknowledge.com/
Kluwer Online
http://www.kluweronline.com/
Taylor & Francis
Group
http://taylorandfrancis.metapress.com/app/home/main.asp
Ingenta
http://www.ingenta.com/
InfoTrieve
http://www4.infotrieve.com/users/logon.asp?component=AF&dk=/search/databases/newsearch.asp
Engineering
& Industry הנדסה ותעשייה
Applied Science &
Technology Index
http://www.hwwilson.com/
Ei Compendex
http://edina.ac.uk/compendex/
Biomedical and Life
Sciences מדעי החיים
PubMed
http://www.ncbi.nlm.nih.gov/PubMed
Medscape (from WebMD)
http://www.medscape.com
BioMedNet
http://www.bmn.com/
Cambridge Scientific Abstracts
http://www.csa.com/
Free Acess
Presentations on the Net מצגות
פתוחות לקהל
Lab
Tech Guy on HPLC wash solvents
Planar
Chromatography
Proteins
– Basics you need to know for
Proteomics
Design
And Purification Of Proteins
High
Performance Liquid Chromatography
High
Performance Liquid Chromatography
HPLC
Mass
Spectrometry
HPLC
Columns and Stationary Phases
The
Basics of Chromatography
HPLC?
Tips
and Tricks: HPLC Troubleshooting
Column
Troubleshooting
Liquid
Chromatography
HPLC
Course - ppt
LC-REsources:
LC-MS extra slides
High
Performance Liquid Chromatography
(HPLC): Haris chapter 25
Analytical
vs Preparative
New
trends in packings, stationary
phases and columns in HPLC
Introduction
Before beginning to work with any HPLC
instrument one must understand the basic
principles of liquid chromatography at
its various modes.
The
liquid chromatograph is very complex and
it consists of sophisticated technology
and sometimes delicate and total
ignorance in the manner it works might
sabotage its parts which are very
expensive. In addition, there are
chemical processes of distribution
between the two phases, the mobile and
stationary, while the analysis goes on,
and there is a need for a minimum
understanding of their theoretical basis
to operate the system correctly.
Also, a minimum understanding of the
method, in which the system performs
data analysis, is needed so that no
false data or results will be obtained
in the analysis.
LISTS OF STEPS NEEDED BEFORE ANY
RUN BY HPLC רשימת צעדים
מקדימים לפני הרצה :
- Filter the solvents with membranes
with cutoff of 0.22-0.45 mM
- Use clean and transparent
reservoirs through which
precipitates and colloids can be
distinguished.
- Make sure that the solvents will
be easily mixed with the previous
solvents in the same inlets (A, B,
C, D). For example methanol or
water should not be placed instead
of hexane directly, or any organic
solvent should not be placed
directly instead of a buffer
reservoir.
- Degass the solvents and purge all
the tubing that lead to the pump.
- Connect the column carefully
according to the flow direction
marked on it (do not connect
directly to the detector).
- Flow the
appropriate solvents through the
column at a low flow-rate (0.1-0.5
ml/min) or reach the composition
gradually using the appropriate
gradient.
- Select the appropriate
wavelength (or other type of
setting) in the detector and wait
for stable baseline
- Prepare the set of methods in the
workstation: Instrument method for
the control on the system,
Processing method for the data
processing and the Report method for
the report of final results.
- When the system and the methods
are ready a blank run should be
performed to test the system and
verify that it is clean from
interferences.
Layout
of the rest of the article:
-Solvent delivery system המשאבה
-Detector הגלאי
-Injector
המזרק
-Gradient
and isocratic גרדיינט או
איזוקרטי
-Data Processing עיבוד נתונים
Getting Started
Solvent
Preparation
)
