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High Performance Liquid Chromatography (HPLC)
                    Lecturer: Dr. Shulamit Levin

3.1.2000

1. (2 hours)         Reversed phase chromatography - Optimization of the Separation   Type of solvent,
                            pH, type of buffer, Ion-pairing additives, temperature.
                            Selection of the appropriate  stationary phase type.

2. (2 hours)         Sample Preparation to HPLC and GC: Solid Phase Extraction (SPE)

3. (2 hours)        Detection in liquid chromatography: General guidelines UV, Fluorescence,
                            Mass-spectrometry,  Refractive Index and Electrochemical detectors , mass spectrometry
                            (LC-MS)  Including: Photo-diode-array (PDA) detectors: Principle of operation, Peak purity,
                            Spectral matching

10.1.2000

4. (1 hours)       Validation of chromatographic methods - System suitability, precision, accuracy, limit of
                             detection, limit of quantification etc.

5. (2 hours)     Scaling up to preprative chromatography (Overloading conditions)  and scaling down
                         to Narrow bore analytical  chromatography down to capillary LC.

6. (1 hours)      Chiral chromatography for the analysis of enantiomers

7. (1 hours)     Analysis of ions - Ion Chromatography (IC) and Capillary electrophoresis.

8. (1 hours)    Analysis of polymers - Gel Permeation Chromatography (GPC) for the determination of
                    molecular weight distribution
 

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