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High Performance Liquid Chromatography (HPLC)
Lecturer: Dr. Shulamit Levin
Ministry of Health Institute of Standards
Wednesdays from 14:30 to 17:30
| Topic No. | Title: |
|---|---|
| 1. | Fundamentals of liquid chromatography |
| 2. | Development of methods in HPLC - various modes of liquid chromatography |
| 3. | Reversed phase chromatography- chemistry and geometry of the columns |
| 4. | Sample Preparation: Solid Phase Extraction (SPE) |
| 5. | Detection in liquid chromatography: General guidelines UV, Fluorescence, Mass-spectrometry, Refractive Index and Electrochemical detectors |
| 6. | Photo-diode-array (PDA) detectors: Principle of operation, Peak purity, Spectral matching |
| 7. | Liquid chromatography - mass spectrometry (LC-MS) for the positive identification of unknown compounds |
| 8. | Gradient conditions - Principles and guidelines |
| 9. | QUANTITATIVE WORK IN HPLC
1 . Peak integration in HPLC - Integration events and calibration methods |
| 10. | 2 . Validation of chromatographic methods - System suitability, precision, accuracy, limit of detection, limit of quantification etc. |
| 11. | 3. Analysis of impurities and degradation products - "working in the ppm world" |
| 12. | Preparative chromatography - considerations in scaling up a separation |
| 13. | System peaks in liquid chromatography - Their origin and formation, how to live with/without them |
| 14. | Chiral chromatography for the analysis of enantiomers |
| 15. | Analysis of ions - Ion Chromatography (IC) and Capillary Ion Analysis (CIA) |
| 16. | Analysis of polymers - Gel Permeation Chromatography (GPC) for the determination of molecular weight distribution |
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Copyright
(C)1997
Dr. Shulamit
Levin
Medtechnica-analytical department
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