- High Performance Liquid Chromatography
(HPLC) is an analytical technique for the separation and determination of
organic and inorganic solutes in any samples especially biological,
pharmaceutical, food, environmental, industrial, etc. In a liquid
chromatographic process a liquid permeates through a porous solid stationary
phase and elutes the solutes into a flow-through detector.
- The stationary phase is usually in the
- form of small-diameter (5-10 mm) uniform particles, packed into a
cylindrical column.
- The typical column is constructed from a rigid material (such as
stainless steel or plastic) and is generally 5-30 cm long and the internal
diameter is in the range of1-9 mm.
- A high pressure pump is required to force the mobile phase through
the column at typical flow rates of 0.1-2 ml/min.
- The sample to be separated is introduced into the mobile phase by
injection device, manual or automatic, prior to the column.
- The detector usually contains low volume cell through which the
mobile phase passes carrying the sample components.
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