"HPLC system"

<body lang=EN-US link=blue vlink=blue style='tab-interval:36.0pt'> <div class=Section1> <p class=MsoNormal><a name=pgtop></a><a href="http://www.forumsci.co.il/HPLC/modes/modesc.htm"><span style='text-decoration:none;text-underline:none'><img border=0 width=24 height=24 id="_x0000_i1025" src="http://www.forumsci.co.il/HPLC/modes/flconts.gif"></span></a><a href="http://www.forumsci.co.il/HPLC/modes/modes2.htm"><span style='text-decoration:none;text-underline:none'><img border=0 width=24 height=24 id="_x0000_i1026" src="http://www.forumsci.co.il/HPLC/modes/flrarrow.gif"></span></a><a href="#pgbot"><span style='text-decoration:none;text-underline:none'><img border=0 width=24 height=24 id="_x0000_i1027" src="http://www.forumsci.co.il/HPLC/modes/flnote.gif"></span></a><a href="mailto:levins@medtechnica.co.il"><span style='text-decoration:none; text-underline:none'><img border=0 width=23 height=24 id="_x0000_i1028" src="http://www.forumsci.co.il/HPLC/modes/flmlsend.gif"></span></a><span style='mso-fareast-language:EN-US'><br> </span><img border=0 width=780 height=540 id="_x0000_i1029" src="http://www.forumsci.co.il/HPLC/modes/modes.gif"><span style='mso-fareast-language: EN-US'><o:p></o:p></span></p> <div class=MsoNormal align=center style='text-align:center'> <hr size=2 width="100%" align=center> </div> <span style='font-size:12.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-US;mso-fareast-language:EN-US; mso-bidi-language:HE'> <ul type=disc> <li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt: auto;mso-list:l0 level1 lfo1;tab-stops:list 36.0pt'><a name=pgbot></a><span style='mso-fareast-language:EN-US'>High Performance Liquid Chromatography (HPLC) is an analytical technique for the separation and determination of organic and inorganic solutes in any samples especially biological, pharmaceutical, food, environmental, industrial, etc. In a liquid chromatographic process a liquid permeates through a porous solid stationary phase and elutes the solutes into a flow-through detector.<o:p></o:p></span></li> <li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt: auto;mso-list:l0 level1 lfo1;tab-stops:list 36.0pt'><span style='mso-fareast-language:EN-US'>The stationary phase is usually in the<o:p></o:p></span></li> <li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt: auto;mso-list:l0 level1 lfo1;tab-stops:list 36.0pt'><span style='mso-fareast-language:EN-US'>form of small-diameter (5-10 mm) uniform particles, packed into a cylindrical column.<o:p></o:p></span></li> <li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt: auto;mso-list:l0 level1 lfo1;tab-stops:list 36.0pt'><span style='mso-fareast-language:EN-US'>The typical column is constructed from a rigid material (such as stainless steel or plastic) and is generally 5-30 cm long and the internal diameter is in the range of1-9 mm.<o:p></o:p></span></li> <li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt: auto;mso-list:l0 level1 lfo1;tab-stops:list 36.0pt'><span style='mso-fareast-language:EN-US'>A high pressure pump is required to force the mobile phase through the column at typical flow rates of 0.1-2 ml/min.<o:p></o:p></span></li> <li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt: auto;mso-list:l0 level1 lfo1;tab-stops:list 36.0pt'><span style='mso-fareast-language:EN-US'>The sample to be separated is introduced into the mobile phase by injection device, manual or automatic, prior to the column.<o:p></o:p></span></li> <li class=MsoNormal style='mso-margin-top-alt:auto;mso-margin-bottom-alt: auto;mso-list:l0 level1 lfo1;tab-stops:list 36.0pt'><span style='mso-fareast-language:EN-US'>The detector usually contains low volume cell through which the mobile phase passes carrying the sample components.<o:p></o:p></span></li> </ul> </span> <div class=MsoNormal align=center style='text-align:center'> <hr size=2 width="100%" align=center> </div> <p class=MsoNormal><a href="#pgtop"><span style='text-decoration:none; text-underline:none'><img border=0 width=24 height=24 id="_x0000_i1032" src="http://www.forumsci.co.il/HPLC/modes/flpage.gif"></span></a><span style='mso-fareast-language:EN-US'><o:p></o:p></span></p> <address><span style='mso-fareast-language:EN-US'>Shulamit Levin </span><a href="mailto:levins@medtechnica.co.il"><span style='mso-fareast-language: EN-US'>levins@medtechnica.co.il</span></a><span style='mso-fareast-language: EN-US'><o:p></o:p></span></address> <p class=MsoNormal><span style='mso-fareast-language:EN-US'><br> </span><span style='font-size:7.5pt;mso-fareast-language:EN-US'>Published by Lotus® Freelance Graphics®</span><span style='mso-fareast-language:EN-US'> <o:p></o:p></span></p> <p><a href="http://www.forumsci.co.il/HPLC/"><span style='text-decoration: none;text-underline:none'><img border=0 width=34 height=33 id="_x0000_i1033" src="http://www.forumsci.co.il/HPLC/modes/exit_anm.gif"></span></a><a href="http://www.forumsci.co.il/HPLC/"><span style='mso-fareast-language: EN-US'>Back to Shula's Main Page</span></a><span style='mso-fareast-language: EN-US'> <o:p></o:p></span></p> <p><a href="http://www.forumsci.co.il/HPLC/topics.html"><span style='text-decoration:none;text-underline:none'><img border=0 width=34 height=33 id="_x0000_i1034" src="http://www.forumsci.co.il/HPLC/modes/exit_anm.gif"></span></a><a href="http://www.forumsci.co.il/HPLC/topics.html"><span style='mso-fareast-language: EN-US'>Back to Topics in Chromatography</span></a><span style='mso-fareast-language: EN-US'> <br>   <br>   <o:p></o:p></span></p> </div> </body>