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<b><font color="#993366"><font size=+1>VARIOUS
MODES OF HPLC - START HERE</font></font></b>
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<li>
High Performance Liquid Chromatography (HPLC) is an analytical technique
for the separation and determination of organic and inorganic solutes in
any samples especially biological, pharmaceutical, food, environmental,
industrial, etc. In a liquid chromatographic process a liquid permeates
through a porous solid stationary phase and elutes the solutes into a flow-through
detector.</li>
<li>
The stationary phase is usually in the</li>
<li>
form of small-diameter (5-10 mm) uniform particles, packed into a cylindrical
column.</li>
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The typical column is constructed from a rigid material (such as stainless
steel or plastic) and is generally 5-30 cm long and the internal diameter
is in the range of1-9 mm.</li>
<li>
A high pressure pump is required to force the mobile phase through the
column at typical flow rates of 0.1-2 ml/min.</li>
<li>
The sample to be separated is introduced into the mobile phase by injection
device, manual or automatic, prior to the column.</li>
<li>
The detector usually contains low volume cell through which the mobile
phase passes carrying the sample components.</li>
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<address>
Shulamit Levin <a href="mailto:levins@medtechnica.co.il">levins@medtechnica.co.il</a></address>
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