Short Course - Advanced Topics in HPLC
Forum
Scientific Education
"Forum Enrichment"
Kfar Hamacabia
14/July/99
Forum
Scientific Education
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Reversed phase:
Stationary phases - the new generation
of packing and particles.
Mobile phases: pH effects and
ion pair operation.
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Solid phase extraction - considerations
and method development.
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Considerations in scaling down to Narrow
bore columns including gradient operation.
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Considerations in scaling up to preparative
work including gradient operation.
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Detection qualification and types of
detectors in HPLC including MS detectors.
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Photo-diode-array (PDA) detectors: Principle
of operation and spectral analysis: Analysis of impurities: Peak purity,
Spectral matching.
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Instrumental and chemical features effecting
the validation of chromatographic methods - System suitability results,
precision, accuracy, limit of detection, limit of quantification etc.
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System peaks in liquid chromatography
- Their origin and formation, how to live with\without them .
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