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Applications Guide for Sep-Pak Cartridges

Sorbent                Surface Character       Suggested Application

 

C18 Red*               Hydrophobic, non-polar  Isolation of hydrophobic

                          bonded silica phase    species from aqueous

                                                 solutions

                                               Drugs & drug metabolites

                                                 in serum, plasma, urine

                                               Peptides in serum, plasma,

                                                 biological fluids

                                               Desalting of peptides, 

                                                 oligonucleotides

                                               Trace organics in environ-

                                                 mental water sample 

                                               Organic acids in wine,

                                                 beverages

tC18 Red*              Hydrophobic, non-polar  Isolation of hydrophobic

                         bonded silica phase     species from aqueous

                                                 solutions

                                               Use for methods involving

                                                 analysis by GC, esp. GC/MS

                                               Use with acidic aqueous

                                                 soluton (pH <= 3)

                                               Polynuclear aromatic

                                                 hydrocarbons, pesticides,

                                                 herbicides in aqueous

                                                 environmental samples

                                                 (EPA Method 525) 

                                               Drugs and drug metabolites

                                                 in serum, plasma, urine

C8 Red*                Hydrophobic, non-polar  Isolation of hydrophobic

                         bonded silica phase     species from aqueous

                                                 solutions

                                               Use for methods requiring

                                                 less retention than C18 

                                               Drugs & drug metabolites

                                                 in serum, plasma, urine

                                               Peptides in serum, plasma 

tC2 Red                Hydrophobic, non-polar  Isolation of hydrophobic

                         bonded silica phase     species from aqueous

                                                 solutions

                                               Use for methods requiring

                                                 less retention than C8

                                               Drugs and drug metabolites

                                                 in serum, plama, urine 

Silica Black*          Hydrophilic, polar      Isolation of low to 

                         neutral phase           moderate polarity species

                                                 from nonaqueous solutions

                                               Alternative to silica gel

                                                 open columns & TLC

                                                 clean-up

                                               Fat soluble vitamins

                                               Pesticides

                                               Lipid classification

                                               Natural products, plant

                                                 pigment

                                               Synthetic organic compounds

Florisil Orange*       Hydrophilic, polar,     Isolation of low to moderate

                         slightly basic phase    polarity species from

                                                 nonaqueous solutions

                                               Official AOAC & EPA Methods

                                                 (Lauric acid value = 90 mg

                                                 per Classic or Plus

                                                 cartridge)

                                               Samples with large amounts

                                                 of fats or lipids

                                               Pesticides, herbicides in

                                                 food, feeds

                                               Polychlorinated biphenyls

                                                 in transformer oil

Alumina A Aqua Green*  Hydrophilic, polar      Isolation of hydrophilic

                         acidic phase            species in nonaqueous

                                                 solutions

                                               Low capacity cation exchange

                                                 (resistant to degradation

                                                 by high activity radio-

                                                 labeled compounds)

                                               Alternative to acidic

                                                 alumina open columns &

                                                 TLC clean-up

                                               Alternative to Official

                                                 AOAC & EPA Methods

                                               Sugars, caffeine in cola

                                                 beverages

                                               Vitamins in feeds, foods

                                               Antibiotics Additives in

                                                 feed 

Alumina N Lime Green*  Hydrophilic, polar      Isolation of hydrophilic 

                         neutral phase           species in nonaqueous

                                                 solutions

                                               Alternative to neutral

                                                 alumina open columns and

                                                 TLC clean-up

                                               Herbicides

                                               Alternative to Official

                                                 AOAC & EPA Methods

                                               Petroleum, synthetic crude

                                                 oil fractionation

                                               Food additives

                                               Synthetic organic compounds 

Alumina B Green*       Hydrophilic, polar      Isolation of hydrophilic

                         basic phase             species in nonaqueous

                                                 solutions

                                               Alternative to basic alumina

                                                 open columns and TLC

                                                 clean-up

                                               Low capacity cation exchange

                                                 (resistant to degradation

                                                 by high activity radio-

                                                 labled compounds)

                                               Alternative to Official AOAC

                                                 & EPA Methods

                                               Pesticides, herbicides, 

                                                 priority pollutant 

                                                 isolation

                                               Steroids 

Accell Plus CM Blue*   Hydrophilic, polar      Isolation of cationic

                         acidic cation           analytes in aqueous or

                         exchange phase          nonaqueous solutions

                                               Alternative to CM cellulose

                                                 and weak cation 

                                                 exchangers.

                                               Pesticide, herbicide,

                                                 priority pollutant

                                                 isolation

                                               Steroids 

Accell Plus QMA        Hydrophilic, polar      Isolation of anionic

  Light Blue*            basic anion             analytes in aqueous or

                         exchange phase          nonaqueous solutions

                                               Alternative to DEAE 

                                                 cellulose and other 

                                                 anion exchangers

                                               Extraction of acidic and

                                                 weakly acidic proteins,

                                                 enzymes

                                               Isolation of immunoglobulins

                                               Peptide pool fractionations

                                               Removal of acidic pigments

                                                 from wines, fruit juices,

                                                 food extracts

                                               Isolation of phenolic

                                                 compounds 

Amino Propyl NH2       Hydrophilic, moder-     Low capacity weak anion

  Yellow*                ately polar             exchanger

                         slightly basic        Drugs and metabolites

                         phase                 Petroleum and lube oil

                                                 fractionation

                                               Saccharides

                                               Phenols and phenolic

                                                 pigments

Cyano Propyl CN        Hydrophobic, moder-     Analytes in aqueous or

  Purple*                ately non-polar         organic solvents 

                         neutral phase         Drugs and metabolites

                                                 in physiological fluids 

                                               Metabolites from mold &

                                                 fermentation products

                                               Pesticides

                                               Hydrophobic peptides 

Diol Tan*              Hydrophobic, moder-     Analytes in aqueous or

                         ately non-polar         organic solvents 

                         neutral phase         Trace elements from water 

                                               Antibiotics from cosmetics

                                               Hydrophobic interaction

                                                 chromatographic isolation

                                                 of peptides, proteins 

DNPH-Silica Gold*      Reagent coated          Formaldehyde, other alde-

                                                 hydes and ketones in air

Ordering Information

Filters and Membranes                          Part No.      

 Syringe 10 mL Luer-Lok(R) w/needle              WAT025172    

 Syringe 100 ?mL                               WAT027621    

 Syringe 25 ?mL                                WAT027622