עבודה כמותית ב- HPLC

אגן כימיקלים 2009-2010

ע"י דר' שולמית לוין, ביופורום

 

Data processing for quantitative work in HPLC in the regulated Lab (4 hours)

 

Standards Considerations

Integration events: Explained and demonstrated

Running sets of samples

Blanks – Carry over and system peaks

System Suitability

Calibration and Quantitation

Parameters effecting quantitation and validation

Requirements from chromatographic data systems

 

UV-VIS Detection Issues (2 hours):

Linearity

Dynamic range

Sensitivity

Maintenance.

 

Origins of problems in Quantitative Results (2 hours):

  Experimental parameters effecting method precision and accuracy

 

Baseline Disturbances

Retention times instabilities

Peak area repeatability