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Selected Topics for Advanced HPLC Users (lectures are in Hebrew)

Forum Scientific Education

25.6.2001

Topic No.
Title: Chemistry of the Separation
Hours
1. Reversed phase: 

Stationary phases - the new generation of packing and particles. 

Mobile phases: pH effects and ion pair operation.
2
2. Sample preparation by solid phase extraction (SPE)- considerations and method development.
2
3. Considerations in scaling down to Narrow bore columns including gradient operation.
2
4. Considerations in scaling up to preparative work including gradient operation.
2

2.7.2001

Topic No.
Title: Detection in HPLC
Hours
1.
  • Detection qualification and types of detectors in HPLC.
    •
    2
    2.
  • Photo-diode-array (PDA) detectors: Principle of operation and spectral analysis: Analysis of impurities: Peak purity, Spectral matching.
    •
    2
    3.
  • Fundamentals of LC-MS operation.
    •
    2
    4.
  • Enhancing sensitivity in HPLC.
    •
    2

    9.7.2001

    Topic No.
    Title: Successfull Operation of HPLC
    Hours
    1. 
  • Considerations in gradient operation
    •
    2
    2. QUANTITATIVE WORK IN HPLC: 
  • Peak integration in HPLC - Integration events and calibration methods
    •
    2
    3.
  • Troubleshooting in HPLC - Chemistry & Instrumentation
    •
    2
    4.
  • System peaks in liquid chromatography - Their origin and formation, how to live with\without them 
    •
    2

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