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Title of Course:
Selected Topics for Advanced HPLC Users (lectures are in Hebrew)

Forum Scientific Education

The following documents are PDF files for viewing and downloading.
If the link does not open, save first the PDF file to your computer hard drive, then open it with Adobe Acrobat Reader.

9/11/2004
Topic No.
Title: Chemistry of the Separation
Hours
1. Reversed phase: 

Stationary phases - the new generation of packing and particles.  Mobile phases: pH effects and ion pair operation.
2

2.		  
Sample preparation by solid phase extraction (SPE)- considerations and method development.

Basic Presentation
2

3.		  
Considerations in scaling down to Narrow bore columns including gradient operation.
2

4.		  
Considerations in scaling up to preparative work including gradient operation.
2
The following documents are PDF files for viewing and downloading.
If the link does not open, save first the PDF file to your computer hard drive, then open it with Adobe Acrobat Reader.

23/11/2004
Topic No.
Title: Detection in HPLC
Hours

1.
Detection qualification and types of detectors in HPLC.
2

2.
Photo-diode-array (PDA) detectors: Principle of operation and spectral analysis: Analysis of impurities: Peak purity, Spectral matching.
2

3.
Fundamentals of LC-MS operation.
2

4.
Enhancing sensitivity in HPLC.
2

The following documents are PDF files for viewing and downloading.
If the link does not open, save first the PDF file to your computer hard drive, then open it with Adobe Acrobat Reader.

30/11/2004
Topic No.
Title: Successfull Operation of HPLC
Hours

1. 
Considerations in gradient operation
2

2.
QUANTITATIVE WORK IN HPLC: 
  • Peak integration in HPLC - Integration events and calibration methods 
  • System Suitability
    •
    2

    3.
    Troubleshooting in HPLC - Chemistry & Instrumentation
    2

    4.
    System peaks in liquid chromatography - Their origin and formation, how to live with\without them
    2

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