Carmel Ollefins course

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Carmel Ollefins
Selected Topics for Advanced HPLC Users (lectures are in Hebrew)

Forum Scientific Education

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Topic No.	Title: Chemistry of the Separation	Hours
1.	Reversed phase: Stationary phases - the new generation of packing and particles. Mobile phases: pH effects and ion pair operation.	2
2.	Detection qualification and types of detectors in HPLC. Photo-diode-array (PDA) detectors: Principle of operation and spectral analysis: Analysis of impurities: Peak purity, Spectral matching.	4
3.	Quantitative Analysis in HPLC Troubleshooting in HPLC - Instrumental and Chemical	2

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