Fundamentals of liquid chromatography - Theory and Instrumentation various modes of liquid chromatography.
Reversed phase chromatography - chemistry and geometry of the columns.
General guidelines UV, PDA, Fluorescence, Mass-spectrometry, Refractive Index and Electrochemical detectors
Gradient conditions - Principles and guidelines
Peak integration in HPLC; Calibration; System suitability; Extraneous Peaks; Validation of chromatographic methods
Troubleshooting in HPLC - Instrumental Considerations
Scouting and Method Development