TOPICS

CHEMISTRY OF THE SEPARATION
 

Fundamentals of liquid chromatography - Theory and Instrumentation various modes of liquid chromatography.

Reversed phase chromatography - chemistry and geometry of the columns. 
 

DETECTION IN LIQUID CHROMATOGRAPHY AND QUALIFICATION OF DETECTORS

General guidelines UV, PDA, Fluorescence, Mass-spectrometry, Refractive Index and Electrochemical detectors

Gradient conditions - Principles and guidelines

QUANTITATIVE WORK IN HPLC

Peak integration in HPLC; Calibration; System suitability; Extraneous Peaks;  Validation of chromatographic methods 

OPERATION AND TROUBLESHOOTING

Troubleshooting in HPLC - Instrumental Considerations

Scouting and Method Development